Throughout the majority of my summer, I spent my time using this instrument in the picture above. What it does is measure absorbance using a software program called Ocean Optics. I would gather data on the concentrations of NADH as enzymatic reactions occurred, which would in return provide me with a change in absorbance inside the cuvette. Next, by this change in absorbance, I was able to calculate what the concentration of certain nucleotides was in the tissue sample provided from the manipulated rats – the rats were separated into specific groups to test how a certain variable would affect the molecular composition of the heart for each group of rats. Once we compiled this data, we were able to make some conclusions on how hypertension affects heart composition in rats.