Cells | Blog #3

Luckily, none of the cells were infected! hooray!! (Although I did have a dream that the cells were…) So I’m going to begin isolating RNA and protein. I watched one of the postdocs how they isolated RNA, and I’m a little sad that it is just a blind following of a kit… I feel like that kind of ruins half of the fun of labwork… the understanding part of labwork. Anyway, at least I’ll be getting some results soon! The protein will be isolated as a crude lysate (just with a lysis buffer reagent, which, again, I googled and the companies will not tell me what is inside). After the lysis, we’ll be running it on a western blot. Hopefully next week some results will come in!

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