Blog #1 | Describe your favorite experience as an intern this summer

I am a research student in a cardiovascular laboratory, and our main goal is to study hypertrophic cardiomyopathy – the most common genetic cardiac disease.  I man all of the immunofluorescence work, which uses antibodies conjugated to fluorescently activated fluorophores to detect proteins of interest in cells/tissue.

Here is an example of the microscopy work that I am working with:

overlay ipsc-cm

This is a cardiomyocyte (heart muscle cell) derived from induced pluripotent stem cells in a wild-type line.  My job is to compare the mutational effects across our different lines to better understand the mechanisms of the disease.

In one of our homozygous truncating mutant lines, I have discovered a unique localization of a protein that no one in the field has seen before (data not shown, confidential to lab).

I love doing immunofluorescence work because it is not only an art, but it allows you to see a direct result of an experiment in an unambiguous, beautiful way.


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